Journal Reviews

Characteristics of limbal lymphatic vasculature on confocal microscopy

4 February 2021 | | Cornea / External Eye Disease | Confocal microscopy, Corneoscleral limbus, Immunofluorescence, Lymphatic vessels

This ex vivo study aims to describe the morphological characteristics of the human limbal lymphatic vasculature using confocal microscopy and immunofluorescence. Twelve corneal specimens are obtained from human donor eyes. These corneal buttons are not suitable for transplantation due to...

Corneal stroma modelling under hypoxic conditions

1 August 2018 | | Cornea / External Eye Disease | Confocal fluorescence microscopy, Cornea, Corneal organ culture, Extracellular matrix, Stroma

The cornea is exposed to hypoxia under several conditions including sleep, inflammation and wound healing. Being an avascular tissue to maintain transparency, how the cornea homeostatic controls oxygen tension is important. To address this process two models were utilised; a...

Stromal cell alignment in damaged cornea

1 August 2017 | | Cornea / External Eye Disease | Confocal microscopy, Corneal wound healing, Extracellular matrix, SHG imaging

Stromal keratocytes are responsible for wound healing in the cornea following damage. Keratocytes at the site of injury become activated and differentiate to myofibroblasts which secrete extracellular matrix (ECM) to close the wound, a response mediated by transforming growth factor...

Non-contact-lens-associated acanthamoeba keratitis

1 October 2015 | | Cornea / External Eye Disease | Acanthamoeba keratitis, microbial keratitis, microscopy, non-contact-lens, time of presentation

This retrospective study was done to study the clinical presentation and microbiological results of non-contact-lens-related acanthamoeba keratitis (AK). One hundred and ninety-four patients with microbiological / histopathological diagnosis of AK over four years were included. All patients had corneal scrapings...

Meibomian gland alteration in patients with primary chronic dacryocystitis: An in vivo confocal microscopy study

1 October 2015 | | Oculoplastics, Orbit | Confocal microscopy, meibomian gland, primary chronic dacryocystitis

Chronic dacryocystitis affects tear film functionality and can predispose to ocular surface disease, however the specific effect of chronic dacryocystitis on meibomian gland function has not been studied. This group therefore performed in vivo confocal microscopy in 28 patients’ eyes...

Autophagy in lens mitochondria loss

1 April 2014 | | Cataract and Refractive | ATG5, FYCO1, FYVE and coiled coil domain containing 1, LC3B, OFZ, Pik3c3, TOM20, autophagy, autophagy-related 5, cataract, differentiation, electron microscopy, lens, microtubule-associated protein 1 light chain 3B, mitophagy, organelle free zone, phosphatidylinositol 3-kinase, catalytic subunit type 3, translocase of the outer mitochondrial membranes 20 kDa

The lens consists of a monolayer of epithelial cells that overlies fibre cells that differentiate from epithelial cells at the equator. While developing, fibre cells need mitochondria to provide energy, as they mature they lose these and other organelles to...