This multicentre retrospective interventional case series reports the outcomes and complications of trabeculotomy ab interno using the Trab360 device in childhood glaucoma’s. Forty-six eyes of 41 patients were included and had a mean follow-up of 14.5 months. Mean preoperative intraocular pressure (IOP) was 30.9mmHg and mean postoperative IOP was 20.3mmHg. Mean number of drops reduced from 2.6 preoperatively to 1.6 postoperatively. Success was defined as IOP less than or equal to 24mmHg with or without medication and no addition surgery and occurred in 67.4% of all eyes. The most favourable outcome was in patients with primary congenital glaucoma at 83.3% but there were only six of these patients. Two eyes (4.3%) suffered cyclodialysis but there were no other significant complications. They concluded that ab interno trabeculotomy with the Trab360 device is effective and safe for treating childhood glaucoma.