Migraine is a common, chronic, multifactorial neuro-vascular disorder typically characterised by recurrent attacks of disabling headache and autonomic nervous system dysfunction (migraine without aura). Up to one third of patients also have neurological aura symptoms (migraine with aura). The objective of this study was to evaluate the retinal nerve fibre layer (RNFL) thickness in migraine patients with or without aura and to search for possible structural effects of migraine on the retina. Parameters related to RNFL thickness of right and left eyes’ average superior, inferior, nasal and temporal quadrant values were found to be similar in migraine and control subjects. When focusing on the RNFL thickness of right and left eyes and the migraine parameters, there were no significant differences between migraine sufferers with and without aura. The overall conclusion from the study was that migraine disease with or without aura does not have any effect on the thickness of the RNFL.