This study aimed to explore the psychometric properties of the CATQoL instrument and to determine the ability of it to measure the impact of amblyopia treatment from a child’s perspective. A total of 342 children were recruited with analysis presented for 331 subjects. Acceptability based on adjusted completion was 91.4% for CATQoL and 86.4% for PEDSQL. Percentage floor and ceiling effects were 0.7 and 0.3%, and 17.5 and 18.5% respectively. Reliability based on internal consistency was 0.793 and 0.872 respectively. Validity evaluation showed a significant moderate correlation between the two questionnaire scores. Amblyopia severity was not significantly affected on the PEDSQL but was significant with the CATQoL. This questionnaire now requires assessment in further independent studies.