This study recruited patients with anisometropic amblyopia in the right eye and without strabismus. This allowed the authors to reduce the effort exerted in cortex activities by different amblyopic eyes. They used whole brain analysis to find the differences between groups. The study included 10 patients aged 10-25 years plus 14 controls aged 9-25 years. The amplitude of low-frequency fluctuation (LFF) was the main results. The amblyopia group produced increased amplitude of LFF results in the left superior temporal gyrus, left inferior parietal lobe, left pons and right inferior semi-lunar lobe along with decreased amplitude of LFF results in two sides of the medial frontal gyrus. There was no significant difference for age or gender. The results demonstrate abnormal spontaneous brain activities in patients with anisometropic amblyopia and these abnormalities may contribute to the neuropathological mechanisms of anisometropic amblyopia.