The authors aimed to evaluate retinal sensitivity and fixation characteristics in children with anisometropic amblyopia by macular analyser integrity assessment (MAIA) microperimetry and to compare the results between amblyopia and fellow eyes. The study included 39 children; 21 female – aged 10 ±2 years. Best corrected visual acuity was 0.31 ±0.16 for the amblyopic eyes and 0.00 ±0.00 for fellow eyes. Mean spherical equivalent refractive error was +1.94 ±5.95 for amblyopic eyes and +2.25 ±2.01D for fellow eyes. Average microperimetry threshold value was significantly decreased for amblyopic versus fellow eyes. The authors conclude retinal sensitivity is significantly higher in non-amblyopic eyes compared to amblyopic. Further, the PI fixation index was significantly higher than amblyopic eyes and correlated significantly with visual acuity. Microperimetry provides information regarding functional status of the macula and fixation pattern. It provides added information about the visual system beyond visual acuity measures.