This cross-sectional study included 71 consecutive acute central serous chorioretinopathy (CSC) patients and 70 age-matched healthy control subjects. Systemic findings, including serum levels of thyroid hormones, thyroid stimulating hormone (TSH), mean arterial pressure (MAP), pulse rate, serum lipid levels and optical coherence tomography findings, were compared between the groups. The mean ages of the CSC and control groups were 41.06 ±6.49 and 40.06 ±7.08-years-old, respectively. Retinal thickness, choroidal thickness, TSH levels, pulse rate and MAP were significantly different between CSC patients and healthy control subjects (range of p values: <0.001–0.042). Using logistic regression analysis, MAP, serum triglyceride concentration and central choroidal thickness were positively associated with CSC (range of p values: <0.001–0.035) The authors report that acute CSC patients had significantly higher pulse rates and MAP and significantly thicker choroidal thickness than were found in healthy subjects. TSH levels were also significantly higher in CSC patients than in controls. However, study parameters were only measured once and the potential of confounding systemic factors cannot be fully excluded. The authors reported an association between hypothyroidism and CSC, and predict that with better ability to discriminate between causative factors involved in CSC we may be able to individualise treatment for patients with CSC.