The goal was to evaluate the use of the Heidelberg anterior segment optical coherence tomography (AS-OCT) to image extraocular rectus muscle insertions and to expand the use of the AS-OCT to also image vertical rectus muscles. The authors evaluated inter and intra examiner repeatability of horizontal and vertical muscle insertion distance measurements to the anatomical limbus. The right eyes of 46 patients without strabismus were measured; mean age of 40.8±17.2 years. Measurements were evaluated for 42 lateral rectus, 43 medial rectus, 35 inferior rectus and 40 superior rectus muscles. Images not captured were because of poor image quality, muscle and limbus not captured in the same image or conjunctival scarring. Excellent correlations (CC>0.75) were found for intra and inter comparisons. Muscle insertion to limbus distances were 6.8±0.7 (lateral rectus), 5.7±0.8 (medial rectus), 6.0±0.6 (inferior rectus) and 6.8±0.6 (superior rectus). The authors conclude the AS-OCT is capable of imaging all rectus muscle insertions with consistent and repeatable measurements.