The aim of this study was to evaluate the efficacy and tolerability of cyclopentolate 1% spray in patients aged three to six years. This was a cross section parallel group study of 61 children. Thirty-one received cyclo 1% drops and 30 received cyclo 1% spray. There were no differences in age and gender for groups, or for iris colour and race. Mean numerical rating scale to evaluate distress levels was 6.4 ±1.2 and 4 ±1.44 respectively which was significant for children <6 years. Pupil diameter was 6.2 and 5.9, and spherical equivalent change was 1.50 versus 1.30D respectively which was not significant. There were no adverse events. Five in the spray group did not have adequate cycloplegia and all had dark irides. The authors conclude that cyclo spray is effective in reducing stress with cyclo administration in young children. However, this was only partially effective in children with dark irides and therefore, drops are preferable for them.