This study reports the use of amniotic membrane in patients with restrictive strabismus or conjunctiva problems during or following complex eye muscle surgery. Ambio 5 was used for replacement of conjunctiva in this retrospective study of 32 cases aged one to 89 years. Of 32 cases, seven had restricted conjunctiva after complex strabismus surgery to extent that transposition was needed. Mean age at surgery was 47 years with mean follow-up of 32 months. The results show that commercially prepared amniotic membrane is an appropriate treatment for large conjunctival defects. It was associated with good functional and subjective outcomes. All patients had improved comfort and / or improved eye movements when in conjunction with extraocular muscle surgery. No case showed rejection of amniotic membrane. Three developed symblepharon which possibly related to the process that makes the amniotic membrane storable.