The study compares central laminar thickness (LT) among patients with glaucomatous optic neuropathy (GON), patients with non-GON and normal subjects. The non-GON group comprised eyes with demyelinating optic neuropathy (ON), anterior ischaemic ON, compressive ON, Leber hereditary ON and traumatic ON. LT was measured using enhanced-depth imaging optical coherence tomography (EDI-OCT). Results showed that LT measurements of GON and non-GON group eyes were thinner than those of control group eyes. In addition, LT of GON group eyes were thinner than those of non-GON group eyes. In the GON group, LTs were thinner in more severely affected eyes. In conclusion, the study results show that EDI-OCT is valuable for documenting structural abnormalities in optic neuropathy. The authors acknowledge that further studies are required to determine the role of OCT in detecting progression of ONs of various types.