The authors sought to determine if the plusoptix would provide an accurate estimation of whether an autistic child had amblyopic risk factors. This retrospective study identified 48 children with autism. Undilated plusoptix was undertaken in 25 children aged less than one year up to 15 years (average six). Amblyopic risk factors were found in 17 children (68%): nine with refractive amblyopia, four with accommodative esotropia, two with exotropia, one with Duane’s retraction syndrome and one with partially accommodative esotropia. Plustopix had sensitivity of 88%, specificity of 87%, false positive of 18%, false negative of 12%, positive predictive value of 94% and negative predictive value of 78%. Plusoptix was found to be useful for screening children with autism.