This study compares amblyopia risk factors in 1) newborns with congenital nasolacrimal duct obstruction (CNLDO) and age-matched healthy controls, 2) newborns with unilateral and bilateral CNLDO and 3) affected eye and fellow eye of newborns with unilateral CNLDO. The authors compared medical records of 151 consecutive patients (aged 44.4 ±9.0 days) with 218 healthy babies (aged 45.8 ±9.6 days). There was no significant difference between groups; 80.8% were unilateral and 29 bilateral cases. Amblyopia risk factors were found in 18 with CNLDO and 19 healthy controls (not significant). This study included only newborns aged 30-60 days. No differences were found in any of the groups considered. The authors conclude CNLDO is not associated with amblyopia risk factors in newborns. However, anisometropia could develop later and thus, follow-up is recommended.